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KMID : 1138520060090020039
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Journal of Pharmacopuncture
2006 Volume.9 No. 2 p.39 ~ p.47
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Effect of Ginseng Radix Rubra Herbal-acupuncture Solution(GRR-HAS) on Gene Expression in SNU484 carcinomar cells
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Won Eun-Ju
Lee Kyung-Min
Lee Bong-Hyo
Lim Seong-Chul
Jung Tae-Young
Seo Jung-Chul
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Abstract
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Objective : It has long been known about the anticancer effect of GRR-HAS, however, it has not been systemically determined the differentially regulated genes by GRR-HAS in cancer cells. The purpose of this study is to screen the GRR-HAS mediated differentially expressed genes in cancer cells such as SNU484 gastric cancer cell lines. Oligonucleotide microarray approache was employed to screen the differential expression genes.
Methods : GRR-HAS was prepared by boiling and stored at -70^{circ}C until use. Cells were treated with various concentrations of GRR-HAS(0.1, 0.5, 1.5, 10, 20mg/ml) for 24 h. Cell toxicity was tested by MTT assay. To screen the differentially expressed genes in cancer cells, cells were treated with 1.5mg/ml of GRR-HAS. For oligonucleotide microarray assay, total RNA was used for gene expression analysis using oligonucleotide Genechip (Human genome Ul33 Plus 2.0., Affimatrix Co.).
Results : It has no cytotoxic effects on both HepG2 and SNU484 cells in all concentrations(0.1, 0.5, 1.5, 10, 20mg/ml). In oligonucleotide microarray assay, in SNU484 cells, the number of more than twofold up-regulated genes was 346. The number of more than twofold down-regulated genes was 9.
Discussion : This study showed the comprehensive gene expression analysis using oligonucleotide microarray for the screening of GRR-HAS mediated differentially regulated genes. These results will provide a better application of GRR-HAS in cancer field and drug target development.
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KEYWORD
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oligonucleotide microarray, CRR-HAS, gene expression, SNU484
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